Journal: Poultry science
Article Title: Inhibitory effect of Eimeria maxima IFN-γ inhibitory molecules on the immune function of T cell subsets in chickens.
doi: 10.1016/j.psj.2023.103098
Figure Lengend Snippet: Figure 2. Detection of the sorting purity of chicken CD8+, CD4+, CD4+ CD25 and CD4+ CD25+ T cells by flow cytometry. (A) Magnetic bead sorting of chicken CD8+ T cells A1: blank cells (total PBMC); A2: CD8+ T cells before cell sorting; A3: CD8+ T cells after cell sorting. (B) Mag- netic bead sorting of chicken CD4+ T cells B1: blank cells (total PBMC); B2: CD4+ T cells before cell sorting; B3: CD4+ T cells after cell sorting. (C) Magnetic bead sorting of chicken CD4+ CD25 T cells C1: blank cells (total PBMC); C2: CD4+ CD25 T cells before cell sorting; C3: CD4+
Article Snippet: The primary phycoerythrin (PE)-conjugated mouse anti-chicken CD8a or CD4 antibody (Southernbiotech, Birmingham, AL) was added according to 1 £ 108 cells.
Techniques: Cytometry, FACS